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KMID : 0382619850050020419
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Hanyang Journal of Medicine
1985 Volume.5 No. 2 p.419 ~ p.431
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Activities and Characteristics of Neutral RNase in Osteosarcoma Tissues
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Abstract
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In the present study activities of neutral and acid ribonucleases (RNases) were determined in malignant tumor tissues in the field of orthopedic surgery including osteogenic sarcoma. Also determined were the positive rates of acid and neutral RNases in the serum to find out whether these enzyme levels could be used as diagnostic marker for osteogenic tomors. Neutral RNases in the osteogenic sarcoma tissue were isolated and analyzed by DEAE-cellulose column chromatography to see whether the enzymes unique to the tumor were pre-sent in the osteogenic sarcoma tissues.
1) Activities of neutral and acid RNases were significantly increased in tumor tissues of osteogenic sarcoma and giant cell tumor as compared with those of control tissues. The degree of increase in the activity was observed to be greater in neutral RNase than in acid RNase.
2) The activity of neutral RNase in serum of patients with osteogenic sarcoma and giant cell tumor was significantly increased and the positive rates of serum neutral RNase as a tumor marker was considerably high in osteogenic sarcoma and giant cell tumor. The activity and the positive rate of acid RNase were, however, significantly low in the two bone tumors. These results suggested that serum neutral RNase activity could be used as a diagnostic marker for osteogenic sarcoma and giant cell tumor.
3) Neutral RNases in osteogenic sarcoma tissue were separated by DEAE-cellulose column chromatography into five peaks for RNases. Of the five peak RNases, at least two RNase peaks were revealed to be unique to the tumor.
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